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rabbit polyclonal anti ccr4 antibody  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal anti ccr4 antibody
    Rabbit Polyclonal Anti Ccr4 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+ccr4/CCR4+Antibody/10__1158_slash_2767___9764__crc___24___0297-105-9-17
    Average 92 stars, based on 4 article reviews
    rabbit polyclonal anti ccr4 antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Incubation:

    Article Title: Pharmacological blockade of fatty acid amide hydrolase (FAAH) by URB597 improves memory and changes the phenotype of hippocampal microglia despite ethanol exposure.
    Article Snippet: .. Sections were incubated overnight at 4 oC in the following diluted primary antibodies: polyclonal rabbit anti-IBA-1 (1:1000, Wako, cat. no. 019-19741), monoclonal mouse anti-iNOS (10 μg/mL, R&D Systems, cat. no. MAB9502), monoclonal mouse antiGFAP (1:500, Sigma-Aldrich, cat. no. G3893), polyclonal rabbit anti-CX3CR1 (1:500, Abcam, cat. no. ab8021), polyclonal rabbit anti-CCR2 (1:300, Novus Biologicals, cat. no. NBP1-78169), polyclonal rabbit anti-CCR4 (1:300, Novus Biologicals, cat. no. NB10056336) and monoclonal rabbit anti-CXCR4 (1:300, Abcam, cat. no. ab124824). .. The following day the sections were washed and incubated in biotinylated donkey anti-rabbit IgG (1:500, GE Healthcare, cat. no. RPN1004) at room temperature for 1.5 h. Then, sections were incubated in ExtrAvidin peroxidase (Sigma-Aldrich) diluted 1:2000 in darkness at room temperature for 1 h. Finally, immunolabeling was revealed with 0.05% diaminobenzidine (DAB, Sigma-Aldrich), 0.05% nickel ammonium sulfate (Ni) and 0.03% H2O2 in PB-saline for 10 min.



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    Depletion of hCcr4a/b or hCaf1a/b inhibits cell proliferation and tumorigenicity. ( A ) Growth curves of the stable knocking down MKN28 cells determined by the MTS assay ( n = 4); ( B ) Growth curves of the AGS cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( C ) Growth curves of the HeLa cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( D ) Tumorigenicity of the MKN28 cells evaluated by the nude mice model. The left panel shows the time-course changes in tumor volume, while the right panel shows the photograph of excised xenografted tumors after inoculation for 2 months ( n = 4). The xenografts of hCaf1a, hCcr4a, and <t>hCcr4b</t> did not result in tumors after inoculation for 2 months (also refer to ), and therefore only the control and sh-hCaf1b groups are presented. * p < 0.05, ** p < 0.01, and *** p < 0.001.
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    Depletion of hCcr4a/b or hCaf1a/b inhibits cell proliferation and tumorigenicity. ( A ) Growth curves of the stable knocking down MKN28 cells determined by the MTS assay ( n = 4); ( B ) Growth curves of the AGS cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( C ) Growth curves of the HeLa cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( D ) Tumorigenicity of the MKN28 cells evaluated by the nude mice model. The left panel shows the time-course changes in tumor volume, while the right panel shows the photograph of excised xenografted tumors after inoculation for 2 months ( n = 4). The xenografts of hCaf1a, hCcr4a, and <t>hCcr4b</t> did not result in tumors after inoculation for 2 months (also refer to ), and therefore only the control and sh-hCaf1b groups are presented. * p < 0.05, ** p < 0.01, and *** p < 0.001.
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    Image Search Results


    Depletion of hCcr4a/b or hCaf1a/b inhibits cell proliferation and tumorigenicity. ( A ) Growth curves of the stable knocking down MKN28 cells determined by the MTS assay ( n = 4); ( B ) Growth curves of the AGS cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( C ) Growth curves of the HeLa cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( D ) Tumorigenicity of the MKN28 cells evaluated by the nude mice model. The left panel shows the time-course changes in tumor volume, while the right panel shows the photograph of excised xenografted tumors after inoculation for 2 months ( n = 4). The xenografts of hCaf1a, hCcr4a, and hCcr4b did not result in tumors after inoculation for 2 months (also refer to ), and therefore only the control and sh-hCaf1b groups are presented. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Journal: Cancers

    Article Title: Human Ccr4 and Caf1 Deadenylases Regulate Proliferation and Tumorigenicity of Human Gastric Cancer Cells via Modulating Cell Cycle Progression

    doi: 10.3390/cancers13040834

    Figure Lengend Snippet: Depletion of hCcr4a/b or hCaf1a/b inhibits cell proliferation and tumorigenicity. ( A ) Growth curves of the stable knocking down MKN28 cells determined by the MTS assay ( n = 4); ( B ) Growth curves of the AGS cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( C ) Growth curves of the HeLa cells with knockdown of deadenylases by siRNA determined by the MTS assay ( n = 3); ( D ) Tumorigenicity of the MKN28 cells evaluated by the nude mice model. The left panel shows the time-course changes in tumor volume, while the right panel shows the photograph of excised xenografted tumors after inoculation for 2 months ( n = 4). The xenografts of hCaf1a, hCcr4a, and hCcr4b did not result in tumors after inoculation for 2 months (also refer to ), and therefore only the control and sh-hCaf1b groups are presented. * p < 0.05, ** p < 0.01, and *** p < 0.001.

    Article Snippet: The anti-hCcr4b rabbit antibody was from OriGene (Rockville, MD, USA).

    Techniques: MTS Assay, Knockdown, Control

    The expression of CCR4. The expression of CCR4 was significantly higher in the test group in all internal times

    Journal: Indian Journal of Dermatology

    Article Title: Evaluation of Lymphocyte Migration to Induced Paederus Dermatitis: An Experimental Study in Rats

    doi: 10.4103/ijd.IJD_447_18

    Figure Lengend Snippet: The expression of CCR4. The expression of CCR4 was significantly higher in the test group in all internal times

    Article Snippet: Following blocking, all sections were subsequently incubated overnight at 4°C with Anti-CD3 Mouse Monoclonal Primary Antibody (1:100 diluted, Santa Cruz, USA), Anti-CCR10 Mouse Monoclonal Primary Antibody (1:200 diluted, Santa Cruz, USA), and Anti-CCR4 Rabbit Polyclonal Primary Antibody (1:100 diluted, Abnova, Taiwan).

    Techniques: Expressing

    The comparison of expression of CD3, CCR4, and CCR10. Based on the results of immunohistochemistry, the expression of CD3, CCR4, and CCR10 in test group at 24, 72, and 120 h compared to the control group showed a significant increase. The peak of expression of all markers was in 72 h after the exposure

    Journal: Indian Journal of Dermatology

    Article Title: Evaluation of Lymphocyte Migration to Induced Paederus Dermatitis: An Experimental Study in Rats

    doi: 10.4103/ijd.IJD_447_18

    Figure Lengend Snippet: The comparison of expression of CD3, CCR4, and CCR10. Based on the results of immunohistochemistry, the expression of CD3, CCR4, and CCR10 in test group at 24, 72, and 120 h compared to the control group showed a significant increase. The peak of expression of all markers was in 72 h after the exposure

    Article Snippet: Following blocking, all sections were subsequently incubated overnight at 4°C with Anti-CD3 Mouse Monoclonal Primary Antibody (1:100 diluted, Santa Cruz, USA), Anti-CCR10 Mouse Monoclonal Primary Antibody (1:200 diluted, Santa Cruz, USA), and Anti-CCR4 Rabbit Polyclonal Primary Antibody (1:100 diluted, Abnova, Taiwan).

    Techniques: Comparison, Expressing, Immunohistochemistry, Control

    Immunohistochemistry staining. (a) CD3 expression after 72 h (x200) (b) CCR4 expression after 72 h (x100) (c) CCR10 expression after 72 h (x100) (d) normal skin (x100)

    Journal: Indian Journal of Dermatology

    Article Title: Evaluation of Lymphocyte Migration to Induced Paederus Dermatitis: An Experimental Study in Rats

    doi: 10.4103/ijd.IJD_447_18

    Figure Lengend Snippet: Immunohistochemistry staining. (a) CD3 expression after 72 h (x200) (b) CCR4 expression after 72 h (x100) (c) CCR10 expression after 72 h (x100) (d) normal skin (x100)

    Article Snippet: Following blocking, all sections were subsequently incubated overnight at 4°C with Anti-CD3 Mouse Monoclonal Primary Antibody (1:100 diluted, Santa Cruz, USA), Anti-CCR10 Mouse Monoclonal Primary Antibody (1:200 diluted, Santa Cruz, USA), and Anti-CCR4 Rabbit Polyclonal Primary Antibody (1:100 diluted, Abnova, Taiwan).

    Techniques: Immunohistochemistry, Staining, Expressing